抗体 > 重组抗体 > N Antibody
  • 产品名称:

    N Antibody

  • 英文名称:

    N Antibody

  • 货号:
    CSB-RA33255A1GMY
  • 规格:
    ¥2,400
  • 别名:
    Nucleocapsid protein, NC, protein N, N
  • 种属:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 免疫原:
    Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
  • 种属反应性:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 应用范围:
    ELISA, WB, GICA; Recommended dilution: ELISA:1:10000-1:50000, WB:1:500-1:5000, GICA:1:1000-1:10000
  • 克隆类型:
    Monoclonal
  • 抗体亚型:
    Mouse scFv fusion with human IgG1 Fc
  • 纯化方式:
    Affinity-chromatography
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:

     


    Western Blot

    Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY) SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY) Secondary Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506) at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 55 kDa



    The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N

    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml.



    In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.


    ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml。 It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1。

  • 说明书:

    注:产品说明书可能有改进,请直接与CUSABIO产品专员联系,索取最新版说明书!

  • Accession No。:
  • 指标名称:
    N (Nucleoprotein)
  • 研究领域:
    Microbiology
  • 克隆号:
    1A6
  • 产品描述:
    Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.
  • ELISA-手册: