抗体 > 单克隆抗体 > CD146 Monoclonal Antibody
  • 产品类型:
    Monoclonal Antibody
  • 产品名称:

    CD146 Monoclonal Antibody

  • 货号:
    CSB-MA013563A0m
  • 规格:
    ¥2,000
  • 种属:
    Human
  • 免疫原:
    Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA)
  • 宿主:
    Mouse
  • 种属反应性:
    Human
  • 应用范围:
    ELISA, WB, IF, FC; Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200
  • 背景:
    Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
  • 克隆类型:
    Monoclonal
  • 抗体亚型:
    IgG2a
  • 纯化方式:
    >95%, Protein G purified
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:


    Western Blot

    Positive WB detected in: MCF-7 whole cell lysate

    All lanes: CD146 antibody at 1:1250

    Secondary

    Goat polyclonal to Mouse IgG at 1/50000 dilution

    Predicted band size: 72, 58 kDa

    Observed band size: 120 kDa



    Western Blot

    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate

    All lanes: CD146 antibody at 1:500

    Secondary

    Goat polyclonal to Mouse IgG at 1/50000 dilution

    Predicted band size: 72, 58 kDa

    Observed band size: 120 kDa



    Immunofluorescence staining of A375 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of Hela cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of MCF-7 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of THP-1 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI。 The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C。 The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)。



    Overlay histogram showing MCF-7 cells stained with CSB-MA013563A0m (red line) at 1:400。 The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C。 The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C。 Isotype control antibody (green line) was used under the same conditions。 Acquisition of >10,000 events was performed。

  • 说明书:

    注:产品说明书可能有改进,请直接与CUSABIO产品专员联系,索取最新版说明书!

  • 别名:
    Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18
  • 克隆号:
    4D10C7