Rat apolipoprotein E (Apo-E) ELISA Kit

Datasheet
  • 中文名称:
    大鼠载脂蛋白E(Apo-E)ELISA Kit
  • 货号:
    CSB-E09749r
  • 规格:
    96T
  • 价格:
    3900
  • 其他:

产品详情

  • 基因名:
    Apoe
  • 别名:
    ApoeApolipoprotein E ELISA kit; Apo-E ELISA kit
  • 缩写:
    APOE
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates, cell culture supernates
  • 检测范围:
    15.6 ng/mL-1000 ng/mL
  • 灵敏度:
    15.6 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-98  
    1:200 Average % 98  
    Range % 93-104  
    1:400 Average % 84  
    Range % 82-88  
    1:800 Average % 90  
    Range % 85-97  
  • 回收率:
    The recovery of rat Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-94  
    EDTA plasma (n=4) 94 90-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    1000 0.067 0.065 0.066    
    500 0.121 0.125 0.123    
    250 0.213 0.204 0.209    
    125 0.386 0.355 0.371    
    62.5 0.604 0.590 0.597    
    31.2 0.811 0.858 0.835    
    15.6 1.109 1.116 1.113    
    0 2.155 2.214 2.185    
  • 货期:
    3-5 working days

引用文献

  • Chernukha IM.et al,Anim Sci J,2018

靶点详情

  • 功能:
    Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
  • 基因功能参考文献:
    1. Infusion of AngII did not lead to the formation of dissecting abdominal aortic aneurysms or atherosclerosis in ApoE-knockout rats.
    2. Results demonstrated that apolipoprotein E4 (ApoE4)exerted direct neurotoxicity and enhanced the neurotoxicity of Abeta1-40 on spatial cognitive function and hippocampal long term potentiation. This may partly elucidate the mechanism by which APOE4 allele exerted negative effects as a major genetic risk factor for developing Alzheimer's disease.
    3. renal denervation improves aortic distensibility and attenuated endothelial dysfunction in ApoE(-/-)-rats.
    4. The expression of ApoE and S-100 correlated with the progression of focal cerebral contusion.
    5. Apolipoprotein E-deficient rats develop atherosclerotic plaques in partially ligated carotid arteries.
    6. rats treated with KD (EXP2) showed a significant down-regulated expression of ZnT-3, MT-3, ApoE, clusterin, and ACAT-1 in hippocampus
    7. Findings suggest that endogenous apoE generates lower density HDL to produce more lipidated HDL using previously synthesized cholesterol through the interaction with ABCA1 in caveolin-1-rich domain of astrocytes
    8. Although rat apolipoprotein E sequence resembles apoE4, an isoform of apoE3, rat apoE displays the biophysical behavior of apoE3.
    9. A potential neuroprotective role of apolipoprotein E-containing lipoproteins through low density lipoprotein receptor-related protein 1 in normal tension glaucoma
    10. Lower expression of MMP-2 and MMP-9 is associated with the increased expression of apoE in the glomerulus
    11. ApoE-containing astrocyte lipoproteins exhibit the most robust interactions with Abeta.
    12. Apolipoproteins E3 exerts neurotrophic and synaptogenic effects in dorsal root ganglion cell cultures.
    13. LpE and LRP1 promote axonal extension, and suggest that lipids exported to LpE by ABCG1 are important for the enhancement of axonal extension mediated by LpE.
    14. ApoE may play a role in lipid rafts or synaptic structural plasticity by the regulation of its levels via dendritic localization and translation.
    15. These observations suggest that ApoE, expressed by activated retinal glia, stimulates RGC neurite outgrowth after intravitreal Zymosan injection.
    16. Although ApoE increases arterial wall accumulation of triglyceride-rich particles(TGRPs), it also reduces the penetration of TGRPs into the arterial wall--a novel antiatherogenic property.
    17. ApoE mRNA was expressed in astrocytes and macrophages, but not in neurons after focal cerebral ischemia
    18. Spatiotemporal changes of apolipoprotein E immunoreactivity and apolipoprotein E mRNA expression after transient middle cerebral artery occlusion in rat brain.
    19. The Apolipoprotein (apo) E is a protein involved in both lipid metabolism and neuroprotection and its expression system will be highly useful for probing the ability of rat apoE to mediate food intake in rats.
    20. Data indicate that the role of apoE4 in cholesterol homeostasis and apolipoprotein cell association is more pronounced in hippocampal neurons.
    21. Our studies suggest that AEC I is not just a simple barrier for gas exchange, but a functional cell that protects alveolar epithelium from injury.
    22. 24(S)-hydroxycholesterol induces apoE-mediated efflux of cholesterol in astrocytes via an LXR-controlled pathway
    23. ApoE expression is not altered during normal brain aging, but there may be a relationship between ApoE and IL-1beta transcription in the cerebral cortex
    24. LRP binds and endocytoses Abeta42 both directly and via apoE but endocytosed Abeta42 is not completely degraded and accumulates in intraneuronal lysosomes
    25. A naturally processed C-terminal ApoE peptide, Ep1.B, has anti-atherogenic activity indicating a role for this naturally metabolized peptide in vascular wound healing and lipid homeostasis
    26. treatment of primary rat mixed glial cell cultures with IL-1beta induced a significant increase in extracellular apoE protein; treatment of primary astrocyte and mixed glial cell cultures with TNF-alpha significantly reduced extracellular apoE
    27. mRNA expression of ApoE in arterial walls was not different between the controls and cerebral aneurysms.
    28. Data demonstrate that apoE suppresses food intake via a mechanism enhancing melanocortin signaling in the hypothalamus.
    29. The finding that hypothalamic apoE and food intake are positively associated during the normal circadian cycle as well as in the period of restricted feeding suggests that hypothalamic apoE is food-entrained and regulates food intake.
    30. Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin.

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  • 亚细胞定位:
    Secreted
  • 蛋白家族:
    Apolipoprotein A1/A4/E family
  • 组织特异性:
    Secreted in plasma.
  • 数据库链接:

    KEGG:

    STRING:

    UniGene:



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