Pig apolipoprotein E (Apo-E) ELISA Kit

Datasheet
  • 中文名称:
    猪载脂蛋白E(Apo-E)ELISA Kit
  • 货号:
    CSB-E17889p
  • 规格:
    96T
  • 价格:
    4200
  • 其他:

产品详情

  • 基因名:
    APOE
  • 别名:
    APOEApolipoprotein E ELISA kit; Apo-E ELISA kit
  • 缩写:
    APOE
  • Uniprot No.:
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    31.25 ng/mL-2000 ng/mL
  • 灵敏度:
    7.81 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:100 Average % 96
    Range % 85-105
    1:200 Average % 90
    Range % 89-98
    1:400 Average % 96
    Range % 82-103
    1:800 Average % 97
    Range % 92-99
  • 回收率:
    The recovery of pig Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 92-105
    EDTA plasma (n=4) 95 85-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average  
    2000 0.131 0.134 0.133  
    1000 0.223 0.226 0.225  
    500 0.335 0.341 0.338  
    250 0.562 0.553 0.558  
    125 0.862 0.860 0.861  
    62.5 1.192 1.214 1.203  
    31.25 1.589 1.607 1.598  
    0 2.417 2.395 2.406  
  • 货期:
    3-5 working days

靶点详情

  • 功能:
    Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
  • 基因功能参考文献:
    1. These data suggest that the -155T>A mutation in the promoter region of the porcine APOE gene is an important functional variant
  • 亚细胞定位:
    Secreted
  • 蛋白家族:
    Apolipoprotein A1/A4/E family
  • 组织特异性:
    Secreted in plasma.
  • 数据库链接:

    KEGG:

    STRING:

    UniGene:



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