Pig Immunoglobulin G(IgG) ELISA Kit

Datasheet
  • 中文名称:
    猪免疫球蛋白G(IgG)ELISA Kit
  • 货号:
    CSB-E06804p
  • 规格:
    96T
  • 价格:
    4200
  • 其他:

产品详情

  • 别名:
    N/A
  • 缩写:
    IgG
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    0.586 μg/mL-150 μg/mL
  • 灵敏度:
    0.467 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 90  
    Range % 85-96  
    1:400 Average % 95  
    Range % 91-107  
    1:800 Average % 93  
    Range % 86-99  
    1:1600 Average % 91  
    Range % 88-97  
  • 回收率:
    The recovery of pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-98  
    EDTA plasma (n=4) 94 89-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    150 0.254 0.251 0.253    
    37.5 0.555 0.517 0.536    
    9.38 0.755 0.721 0.738    
    2.34 0.999 0.981 0.990    
    0.58 1.169 1.113 1.141    
    0 1.481 1.465 1.473    
  • 货期:
    3-5 working days

引用文献

  • GD Satessa,animals,2020
  • Shao Y,Front Microbiol,2020
  • Satessa GD,Animals (Basel),2020
  • Zong X, et al,Livestock Science,2018
  • JunChen, et al,Journal of trace elements in medicine and biology,2018
  • Yang CM, et al,Journal of Animal Science,2018
  • Peng Liao.et al,Food and Agricultural Immunology ,2017
  • Peng Liao.et al,Food and Agricultural Immunology ,2017
  • Xiangfang Zeng.et al,Livestock Science,2015
  • Wu S et al,Peptides,2012
  • Fengrui Zhang et al,PLoS One,2013


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