Human Folic acid(FA) ELISA Kit

Datasheet
  • 中文名称:
    人叶酸(FA) ELISA Kit
  • 货号:
    CSB-E17109h
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 别名:
    N/A
  • 缩写:
    FA
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    12.5 pg/mL-200 pg/mL
  • 灵敏度:
    6.25 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 89  
    Range % 84-93  
    1:40 Average % 93  
    Range % 89-97  
    1:80 Average % 95  
    Range % 90-100  
    1:160 Average % 84  
    Range % 80-88  
  • 回收率:
    The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-105  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.093 0.090 0.092    
    100 0.227 0.208 0.218    
    50 0.378 0.367 0.373    
    25 0.618 0.637 0.628    
    12.5 0.986 1.003 0.995    
    0 1.485 1.429 1.457    
  • 货期:
    3-5 working days

引用文献

  • Nesreen Saadeh.et al,BIOMEDICAL REPORTS,2018
  • Laila Rashed.et al,Journal of Oral Pathology&Medicine,2017
  • Sun K.et al,J Thromb Thrombolysis.,2016

问答及客户评论

 常见问题解答
Q:

We are looking for Elisa kit for human folic acid, detection range: 310-1000 nmol/L.
Material: erythrocytes (not full blood)
Please tell me is the result obtained from your kit can be comparable to the microbiological reference method?

A:
Thank you for your enquiry!Currently we just have this kit, and the detailed information, pls check the manual.
The theory of different detection methods are different.Since we have no information which is comparable to the microbiological reference method.
Pls just take your own actual test result.


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